bronchitis with expectoration

Brassica rapa

(In territories with significant traditional TRAMIL use)

Dominica:

  • turnip
Significant uses found by the TRAMIL surveys

  root, syrup (decoction + sugar), orally1

Recommendations Preparation and Dosage References

According to published and other information:

Use for pneumonia is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, and on available published scientific information.

Due to health risks associated with pneumonia, an initial medical evaluation is recommended.  The use of this resource can be considered complementary to medical treatment, unless contraindicated.

Should there be a notable worsening of the patient’s condition, or should the respiratory disorder last more than 5 days, seek medical attention.

Not for use by women intending to become pregnant, during pregnancy, during lactation or by children under 5 years old, or by patients with thyroidal disease.

It should not be used as a therapeutic resource for more than 7 consecutive days.

The seed should not be ingested, due to toxicity risk.

The leaf and the root of Brassica rapa are widely used for human consumption.

For “pneumonia”:

Prepare a syrup from a decoction made with 50 grams of ground fresh root in 500 mL (2 cups large glasses or one pint) of water, boil for at least 10 minutes in a covered pot, filter, add 850 grams of sugar and mix until a translucent solution is obtained, pour into a container and fill with water to make up one liter (two pints).

1 CHARLES C, 1988
TRAMIL survey. Movement for Cultural Awareness MCA, Roseau, Dominica.

2 TSUMURAYA Y, NAKAMURA K, HASHIMOTO Y, YAMAMOTO S, 1984
Immunological properties of arabinogalactan proteins from leaves of cruciferous plants. Agr Biol Chem 48(11):2915-2917.

3 GUSTINE DL, 1985
Determination of S-methyl cysteine sulfoxide in Brassica extracts by high-performance liquid chromatography. J Chromatogr 319(3):450-453.

4 IGARASHI K, ABE S, SATOH J, 1990
Effects of atsumi-kabu (red turnip, Brassica campestris L.) anthocyanin on serum cholesterol levels in cholesterol-fed rats. Agr Biol Chem 54(1):171-175.

5 YAO G, LI YJ, CHANG XQ, LU J, 1983
Vitamin C content in vegetables and fruits in Shenyang (China) market during four seasons. Yingyang Xuebao 5(4):373-379.

6 TAWFIQ N, HEANEY RK, PLUMB JA, FENWICK GR, MUSK SR, WILLIAMSON G, 1995
Dietary glucosinolates as blocking agents against carcinogenesis: glucosinolate breakdown products assessed by induction of quinone reductase activity in murine hepa1c1c7 cells. Carcinogenesis 16(5):1191-1194.

7 DUKE JA, 1992
Handbook of biologically active phytochemical constituents of GRAS herbs and other economic plants. Boca Raton, USA: CRC Press.

8 DUKE JA, ATCHLEY AA, 1986
Handbook of proximate analysis tables of higher plants. Boca Raton, USA: CRC Press. p30.

9 EL-SAYED AM, EL-SAKHEWY FS, 1993
Furochromone and thiooxazolidone principles of Brassica rapa (Turnip). Az J Pharm Sco 11(6):11-20.

10 BADRIA FA, 1994
Is man helpless against cancer? An environmental approach: antimutagenic agents from Egyptian food and medicinal preparations. Cancer Lett 84(1):1-5.

11 MORITA K, HARA M, KADA T, 1978
Studies on natural desmutagens: Screening for vegetable and fruit factors active in inactivation of mutagenic pyrolysis products from amino acids. Agr Biol Chem 42(6):1235-1238.

12 KADA T, MORITA K, INOUE T, 1978
Anti-mutagenic action of vegetable factor(s) on the mutagenic principle of tryptophan pyrolysate. Mutat Res 53(3):351-353.

13 YAMAGUCHI T, YAMASHITA Y, ABE T, 1980
Desmutagenic activity of peroxidase on autoxidized linolenic acid. Agr Biol Chem 44(4):959-961.

14 KRAMER J, MAHADEVAN S, HUNT JR, SAUER FD, CORNER AH, CHARLTON KM, 1973
Growth rate, lipid composition, metabolism and myocardial lesions of rats fed rapeseed oils (Brassica campestris var. arlo, Echo and Span, and B. napus var. oro.) J Nutr 103(12):1696-1708.

15 KRAMER J, FRIEND D, HULAN H, 1975
Lipid changes in tissue of young boars fed rapeseed oil or corn oil. Nutr Metab 19(5-6):279-290.

16 KRAMER JK, HULAN HW, MAHADEVAN S, SAUER FD, CORNER AH, 1975
Brassica campestris var. span: II. Cardiopathogenicity of fractions isolated from span rapeseed oil when fed to male rats. Lipids 10(9):511-516.

17 GRANDHI RR, SLINGER SJ, SUMMERS JP, 1977
Productive performance and liver lesions in two strains of laying hens receiving two rapeseed meals. Poult Sci 56(6):1904-1908.

18 SARKAR SR, SINGH LR, UNIYAL BP, MUKHERJEE SK, NAGPAL KK, 1983
Effect of common vegetables on thyroid function in rats. A preliminary study. Def Sci J 33(4):317-321.

19 GREER MA, ASTWOOD EB, 1948
The antithyroid effect of certain foods in man as determined with radioactive iodine. Endocrinology 43:105-119.

Lippia graveolens

(In territories with significant traditional TRAMIL use)

Costa Rica:

  • orégano
  • wild marjoran
Significant uses found by the TRAMIL surveys

  leaf and stem, decoction, orally1

Recommendations Preparation and Dosage References

According to published and other information:

Use for bronchitis with expectoration is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, toxicity studies and available published scientific information.

The leaf and the stem ofLippia graveolens are widely used as a spice for human consumption.

For bronchitis with expectoration:

There is no available information establishing a means of preparation and dosage other than that referred to by traditional use.

1 OCAMPO R, 1988
Encuesta TRAMIL (Costa atlántica), Instituto de Desarrollo Agrario, Universidad de Costa Rica, San José, Costa Rica.

2 DOMINGUEZ X, SANCHEZ H, SUAREZ M, BALDAS J, GONZALEZ M, 1989
Chemical constituents of Lippia graveolens. Planta Med 55(2):208-209.

3 PINO J, ROSADO A, BALUJA R, BORGES P, 1989
Analysis of the essential oil of Mexican oregano. Die Nahrung 33(3):289-295.

4 COMPADRE C, HUSSAIN R, LEON I, ENRIQUEZ R, 1987
Volatile constituents of Montanoa tomentosa and Lippia graveolens. Planta Med 53(5):495-496.

5 SALGUEIRO LR, CAVALEIRO C, GONCALVES MJ, PROENCA DA CUNHA A, 2003
Antimicrobial activity and chemical composition of the essential oil of Lippia graveolens from Guatemala. Planta Med 69(1):80-83.

6 DABROY LP, 1994.
Confirmación de la actividad antibacteriana de algunas especies del género Lippia contra bacterias que causan infección respiratoria. Tesis Facultad de Ciencias Químicas y Farmacia. Universidad San Carlos de Guatemala, Guatemala.

7 MENDOZA JC, 1995.
Confirmación de la actividad antimicrobiana de 3 especies del género Lippia. Tesis Facultad de Ciencias Químicas y Farmacia. Universidad San Carlos de Guatemala, Guatemala.

8 CACERES A, SALVADOR L, 1994.
Actividad antibacteriana y antifúngica de plantas de uso medicinal en Guatemala. Memoria del Congreso Científico. 10 años del CYTED. Cancún, México.

9 GARCIA GM, COTO MT, GONZALEZ CS, PAZOS L, 2002
Toxicidad aguda en ratones, del extracto acuoso de hoja y tallo de Lippia graveolens. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Universidad de Costa Rica, San Pedro, Costa Rica.

Mangifera indica

(In territories with significant traditional TRAMIL use)

Saint Lucia:

  • mango

Guatemala:

  • mango

Haiti:

  • mango
Significant uses found by the TRAMIL surveys
Recommendations Preparation and Dosage References

According to published and other information:

Use for indigestion (burn) is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, toxicity studies, scientific validation and available published scientific information.

The use of the flower for bronchitis is classified as REC, based on the significant traditional use documented in the TRAMIL surveys and toxicity studies.

The use of the flower for weakness, pneumonia and cough is classified as REC, based on the significant traditional use documented in the TRAMIL surveys and toxicity studies.

Should there be a notable worsening of the patient’s condition, or should bronchitis, pneumopathy or cough last more than 2 days, seek medical attention.

The fruit ofMangifera indica is widely used for human consumption.

For cough and pneumonia:

Prepare a decoction with 15-20 leaves in 1 liter of water, boil for at least 10 minutes in a covered pot, allow to cool, and drink 1 cup 3 times a day.

For bronchitis, indigestion (burn) and weakness:

Prepare an infusion, adding 250 mL (1 cup) of boiling water to 3 leaves.  Cover and allow cool down for 5-10 minutes, and then filter.  Drink 1 cup 3 times a day.

1 GIRON L, 1988
Encuesta TRAMIL (Costa atlántica). Centro Mesoamericano de Tecnología CEMAT, Guatemala, Guatemala.

2 JEAN-PIERRE L, 1988
TRAMIL survey. St. Lucia national herbarium, Castries, St. Lucia

3 WENIGER B, ROUZIER M, 1986
Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti.

4 CRAVEIRO AA, ANDRADE CH, MATOS FJ, ALENCAR JW, MACHADO MI, 1980
Volatile constituents of Mangifera indica Linn. Rev Latinoamer Quim 11:129.

5 TANAKA T, SUEYASU T, NONAKA G-I, NISHIOKA I, 1984
Tannins and related compounds. XXI. Isolation and characterization of galloyl and p-hydroxybenzoyl esters of benzophenone and xanthone c-glucosides from Mangifera indica L. Chem Pharm Bull 32(7):2676-2686.

6 PROCTOR JTA, CREASY LL, 1969
The anthocyanin of the mango fruit. Phytochemistry 8(10):2108.

7 SHAFT N, IKRAM M, 1982
Quantitative survey of rutin-containing plants. Part 1. Int J Crude Drug Res 20(4):183-186.

8 NIGAM IC, 1962
Studies in some Indian essential oils. Agra Univ J Res Sci 11:147-152.

9 LU ZY, MAO HD, HE MR, LU SY, 1982
Studies on the chemical constituents of mangguo (Mangifera indica) leaf. Chung Ts'ao Yao 13:3-6.

10 PHARM XS, PHARM GK, 1991
The extraction and determination of the flavonoid mangiferin in the bark and leaves of Mangifera indica. Tap Chi Duoc Hoc 5:8-19.

11 ANJANEYULU V, PRASAD KH, RAO GS, 1982
Triterpenoids of the leaves of Mangifera indica. Indian J Pharm Sci 44:58-59.

12 GRIFFITHS LA, 1959
On the distribution of gentisic acid in green plants. J Exp Bot 10(3):437-442.

13 GHOSAL S, BISWAS K, CHATTOPADHYAY BK, 1978
Differences in the chemical constituents of Mangifera indica infected with Aspergillus niger and Fusarium moniliformae. Phytochemistry 17(4):689-694.

14 KHAN MA, KHAN MNI, 1989
Alkyl gallates of flowers of Mangifera indica. Fitoterapia 60(3):284.

15 KHAN MA, KHAN MNI, 1993
Studies in the chemical constituents of flowers of Mangifera indica. Part-II. Isolation and characterization of some alkylgallates from blossoms of Mangifera indica. Pak J Sci Ind 35(7/8):276-278.

16 DUKE JA, ATCHLEY AA, 1986
Handbook of proximate analysis tables of higher plants. Boca Raton, USA: CRC Press. p105.

17 GARCIA GM, COTO MT, GONZALEZ CS, PAZOS L, 1998
Velocidad del tránsito intestinal en ratón, del extracto acuoso de hoja fresca de Mangifera indica. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

18 CACERES A, GONZALEZ S, GIRON L, 1998
Demostración de la actividad antimicrobiana de plantas tramil en base a los usos populares en la cuenca del Caribe. Informe TRAMIL. Laboratorio de productos fitofarmacéuticos Farmaya y Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala, Guatemala.

19 GARCIA GM, COTO MT, GONZALEZ CS, PAZOS L, 1999
Actividad bronquial del extracto acuoso de flores frescas de Mangifera indica. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

20 SOUZA BRITO ARM, HIRUMA-LIMA CA, LIMA ZP, 2003
Atividades biológicas obtidas dos extratos hidroalcoólicos das folhas e flores da Mangifera indica. Informe TRAMIL, Depto. Fisiologia, Inst. Biociências UNESP, Botucatu, SP y Depto. Fisiologia, Inst. Biologia, UNICAMP, Campinas, Sao Paulo, Brasil.

21 ASWAL BS, BHAKUNI DS, GOEL AK, KAR K, MEHROTRA BN, MUKHERJEE KC, 1984
Screening of Indian plants for biological activity: Part X. Indian J Exp Biol 22(6):312-332.

22 HERRERA J, 1992
Determinación de parámetros farmacológicos usados en medicina tradicional popular en la cuenca del Caribe. Informe TRAMIL. Dep. de Farmacología, Facultad de Salud, Universidad del Valle, Cali, Colombia.

23 GARCIA GM, COTO MT, GONZALES CS, PAZOS L, 2000
Toxicidad aguda en ratones, del extracto acuoso de flores frescas de Mangifera indica. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

24 FRAME AD, RIOSOLIVARES E, DE JESUS L, ORTIZ D, PAGAN J, MENDEZ S, 1998
Plants from Puerto Rico with anti-Mycobacterium tuberculosis properties. P R Health Sci J 17(3):243-253.

25 SHARMA SR, DWIVEDI SK, SWARUP D, 1997
Hypoglycaemic potential of Mangifera indica leaves in rats. Int J Pharmacog 35(2):130-133.

26 OLIVER-BEVER B, 1986
Medicinal plants in tropical West Africa. Cambridge, USA: Cambridge University Press.

27 GUPTA MP, ARIAS TD, CORREA M, LAMBA SS, 1979
Ethnopharmacognostic observations on Panamanian medicinal plants. Part I. Q J Crude Drug Res 17(3/4):115-130.

Persea americana

(In territories with significant traditional TRAMIL use)

Mexico:

  • aguacate

Guatemala:

  • aguacate

Dominican Republic:

  • aguacate

Barbados:

  • pear tree

Martinique:

  • zaboka
Significant uses found by the TRAMIL surveys

  leaf, decoction, orally2

Recommendations Preparation and Dosage References

According to published and other information:

Use for amenorrhea is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, toxicity studies, scientific validation and available published scientific information.

Use for asthma, bronchitis, flatulence, urinary infection and cough is classified as REC, based on the significant traditional use documented in the TRAMIL surveys and toxicity studies.

Should there be a notable worsening of the patient’s condition, or should asthma, bronchitis or cough last more than 5 days, or should urinary infection persist for more than 3 days, seek medical attention.

Due to the risks of documented interactions with warfarin and monoamine-oxidase inhibitors (MAOI), ingestion of the fruit decoction should be avoided by persons taking these medicines5.

Not for use during lactation or by children under 3 years old.

 

Not for use during pregnancy because it may have abortifacient effect.

The fruit of Persea americana is widely used for human consumption.

For amenorrhea, asthma, bronchitis, flatulence, urinary infection and cough:

Prepare a decoction with 20 grams (3 spoonfuls) of ground leaf in 1 liter (4 cups) of water, boil for at least 10 minutes in a covered pot.  Filter, allow to cool and drink 1/2-1 cup 3-4 times a day26.

Any medicinal preparation must be preserved cold and used within the 24 hours.

1 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984
Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana.

2 GIRON L, 1988
Encuesta TRAMIL (Costa atlántica). Centro Mesoamericano de Tecnología CEMAT, Guatemala, Guatemala.

3 FAUJOUR A, MURREY D, CHELTENHAM-CORBIN B, CARRINGTON S, 2003
TRAMIL survey. enda-caribbean, IICA & UAG, Saint Thomas, Barbados.

4 LONGUEFOSSE JL, NOSSIN E, 1990-95
Enquête TRAMIL. Association pour la valorisation des plantes médicinales de la Caraïbe AVPMC, Fort de France, Martinique.

5 MENDEZ M, MEDINA ML, DURAN R, 1996
Encuesta TRAMIL. Unidad de recursos naturales, Centro de Investigación Científica de Yucatán CICY, Mérida, México.

6 CANIGUERAL S, VILA R, RISCO E, PEREZ F, PORTILLO A, FREIXA B, MILO B, VANACLOCHA B, RIOS JL, MORALES MA, ALONSO JR, BACHILLER LI, PERIS JB, STUBING G, 2002
Persea americana. Vademecum de Fitoterapia, Editorial Masson, Barcelona, España, Feb. 26, 2003. URL: www.masson.es/book/fitoterapia.html

7 BERGH BO, SCORA RW, STOREY WB, 1973
Comparison of leaf terpenes in Persea subgenus persea. Bot Gaz (Chicago) 134(2)130-134.

8 KING JR, KNIGHT RJ, 1992
Volatile components of the leaves of various avocado cultivars. J Agric Food Chem 40(7):1182-1185.

9 DE ALMEIDA AP, MIRANDA MMFS, SIMONI IC, WIGG MD, LAGROTA MHC, COSTA SS, 1998
Flavonol monoglycosides isolated from the antiviral fractions of Persea americana (Lauraceae) leaf infusion. Phytother Res 12(8):562-567.

10 MERIÇLI F, MERIÇLI AH, YILMAZ F, YÜNCÜLER G, YÜNCÜLER O, 1992
Flavonoids of avocado (Persea americana) leaves. Acta Pharm Turc 34(2):61-63.

11 BATE-SMITH EC, 1975
Phytochemistry of proanthocyanidins. Phytochemistry 14(4):1107-1113.

12 MURAKOSHI S, ISOGAI A, CHANG CF, KAMIKADO T, SAKURAI A, TAMURA S, 1976
The effects of two components from avocado leaves (Persea americana) and related compounds on the growth of silkworm larvae, Bombyx mori. Nippon Oyo Dobutsu Konchu Gakkaishi 20:87-91.

13 CACERES A, GONZALEZ S, GIRON L, 1998
Demostración de la actividad antimicrobiana de plantas TRAMIL en base a los usos populares en la cuenca del Caribe. Informe TRAMIL. Laboratorio de productos fitofarmacéuticos Farmaya y Facultad de ciencias químicas y farmacia, Universidad de San Carlos, Guatemala, Guatemala.

14 HERRERA J, 1986
Determinación de actividades biológicas de vegetales utilizados en medicina tradicional. Informe TRAMIL. Dep. de Farmacología, Facultad de Salud, Universidad del Valle, Cali, Colombia.

15 GARCIA GM, COTO MT, GONZALEZ CS, PAZOS L, 1999
Actividad bronquial del extracto acuoso de hoja fresca de Persea americana. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

16 PAZOS L, COTO T, GONZALEZ S, QUIROS S, 2003
Tránsito intestinal, en ratones, del extracto acuoso de hojas frescas de Persea americana. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

17 MORON FJ, GARCIA AI, VICTORIA MC, MOREJON Z, LOPEZ M, BACALLAO Y, FUENTES V, 2008
Acción analgésica de la decocción de hojas frescas de Persea americana Mill. (aguacate) en ratones. Trabajo TRAMIL. Laboratorio Central de Farmacología, Facultad de Medicina “Dr. Salvador Allende”, La Habana, Cuba.

18 ADEYEMI OO, OKPO SO, OGUNTI OO, 2002
Analgesic and anti-inflammatory effects of the aqueous extract of leaves of Persea americana Mill Lauraceae. Fitoterapia 73(5):375-380.

19 HERRERA J, 1988
Determinación de actividades biológicas de vegetales utilizados en medicina tradicional. Informe TRAMIL. Laboratorio de fitofarmacología, Dep. de Farmacología, Facultad de Salud, Universidad del Valle, Cali, Colombia.

20 GARCIA GM, COTO MT, GONZALEZ CS, PAZOS L, 2000
Toxicidad aguda en ratones, del extracto acuoso de hojas frescas de Persea americana. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

21 LOPEZ M, MARTINEZ MJ, MOREJON Z, BOUCOURT E, FUENTES V, MORON F. 2005
Irritabilidad dérmica primaria de hoja fresca machacada de Persea americana Mill. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Medicina “Dr. Salvador Allende”, La Habana, Cuba.

22 CRAIGMILL AL, SEAWRIGHT AA, MATTILA T, FROST AJ, 1989
Pathological changes in the mammary gland and biochemical changes in milk of the goat following oral dosing with leaf of the avocado (Persea americana). Aust Vet J 66(7):206-211.

23 GRANT R, BASSON PA, BOOKER HH, HOFHERR JB, ANTHONISSEN M, 1991
Cardiomyopathy caused by avocado (Persea americana Mill.) leaves. J S Afr Vet Assoc 62(1):21-22.

Sphagneticola trilobata

(In territories with significant traditional TRAMIL use)

Puerto Rico:

  • manzanilla

Costa Rica:

  • marigold

Grenada:

  • zeba femme
Significant uses found by the TRAMIL surveys

  aerial parts, decoction, orally1

Recommendations Preparation and Dosage References

According to published and other information:

Use for bronchitis is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, toxicity studies, scientific validation and available published scientific information.

Uses for menstrual pain and vomiting are classified as REC, based on the significant traditional use (OMS/WHO)4 documented in the TRAMIL surveys.

For bronchitis, menstrual pain and vomiting:

There is no available information establishing a means of preparation and dosage other than that referred to by traditional use.

Any medicinal preparation must be preserved cold and used within the 24 hours.

1 OCAMPO R, 1988
Encuesta TRAMIL (Costa atlántica), Instituto de Desarrollo Agrario, Universidad de Costa Rica, San José, Costa Rica.

2 MARCELLE G, 1996
TRAMIL survey. Produce chemist laboratory, Ministry of Agriculture, St George’s, Grenada.

3 BENEDETTI MD, 1994
Encuesta TRAMIL. Universidad de Puerto Rico, Mayagüez, Puerto Rico.

4 BOHLMANN F, ZIESCHE J, KING RM, ROBINSON H, 1981
Naturally occurring terpene derivatives. Part 300. Eudesmanolides and diterpenes from Wedelia trilobata and an ent-kaurenic acid derivative from Aspilia parvifolia. Phytochemistry 20(4):751-756.

5 BOHLMANN F, NGO LE VAN, 1977
Naturally occurring terpene derivatives. 97. New kaurene derivatives from Wedelia species. Phytochemistry 16(5):579-581.

6 CRAVEIRO AA, MATOS FJA, ALENCAR JW, MACHADO MIL, KRUSH A, SILVA MGV, 1993
Volatile constituents of two Wedelia species. J Essent Oil Res 5(4):439-441.

7 KOHEIL MA, 2000
Study of the essential oil of flower-heads of Wedelia trilobata (L.) Hitch. Al-Azhar J Pharm Sci 26:288-293.

8 CÁCERES A, GONZÁLEZ S, GIRÓN L, 1998
Demostración de la actividad antimicrobiana de plantas TRAMIL en base a los usos populares en la cuenca del Caribe. Laboratorio de productos fitofarmacéuticos Farmaya y Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala, Guatemala.

9 CÁCERES A, GONZÁLEZ S, GIRÓN L, 2000
Demostración de la actividad antimicrobiana de plantas TRAMIL en base a los usos populares en la cuenca del Caribe. Laboratorio de productos fitofarmacéuticos Farmaya y Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala, Guatemala.

10 GARCÍA GM, COTO MT, GONZÁLEZ CS, PAZOS L, 1999
Actividad bronquial del extracto acuoso de planta entera fresca de Wedelia trilobata. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

11 PAZOS L, COTO MT, GONZÁLEZ CS, QUIROS S, 2003
Tránsito intestinal, en ratones, del extracto acuoso de hojas frescas de Wedelia trilobata. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Universidad de Costa Rica, San Pedro, Costa Rica.

12 GARCÍA GM, COTO MT, GONZÁLEZ CS, OCAMPO R, PAZOS L, 2001
Velocidad del tránsito intestinal en ratones, del extracto acuoso de brotes frescos de Wedelia trilobata. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBi, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica.

13 HERRERA J, 1992
Determinación de parámetros farmacológicos usados en medicina tradicional popular en la Cuenca del Caribe. Departamento de Farmacología, Facultad de Salud, Universidad del Valle, Cali, Colombia.

14 GOH CL, 1986
Contact sensitivity to Wedelia trilobata. Contact Dermatitis 14(2):126.